Literature summary for 3.1.2.12 extracted from

Miller, J.J.; Shah, I.T.; Hatten, J.; Barekatain, Y.; Mueller, E.A.; Moustafa, A.M.; Edwards, R.L.; Dowd, C.S.; Planet, P.; Muller, F.L.; Jez, J.; Odom John, A.R.
Structure-guided microbial targeting of antistaphylococcal prodrugs (2021), eLife, 10, e66657 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
structure to 1.6 A resolution. A single dimer of FrmB is observed in the asymmetric unit. The overall fold of FrmB is characteristic of the alpha/beta-hydrolase fold	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	Q2FUY3	-	-
Staphylococcus aureus PS 47	Q2FUY3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-D-lactoylglutathione + H2O	-	Staphylococcus aureus	D-lactate + glutathione	-	?
(S)-D-lactoylglutathione + H2O	-	Staphylococcus aureus PS 47	D-lactate + glutathione	-	?
bis(pivaloyloxymethyl)(1-hydroxy-2-oxopiperidin-3-yl)phosphonate + H2O	i.e. POM-HEX, substrate is a prodrug of the compound HEX,which inhibits the glycolytic enzyme enolase	Staphylococcus aureus	[[(hydroxymethoxy)(1-hydroxy-2-oxopiperidin-3-yl)phosphoryl]oxy]methyl 2,2-dimethylpropanoate + 2,2-dimethylpropanoic acid	-	?
bis(pivaloyloxymethyl)(1-hydroxy-2-oxopiperidin-3-yl)phosphonate + H2O	i.e. POM-HEX, substrate is a prodrug of the compound HEX,which inhibits the glycolytic enzyme enolase	Staphylococcus aureus PS 47	[[(hydroxymethoxy)(1-hydroxy-2-oxopiperidin-3-yl)phosphoryl]oxy]methyl 2,2-dimethylpropanoate + 2,2-dimethylpropanoic acid	-	?
fluorescein-3',6'-bis(oxy)methylene bis(2-methoxypropanoate) + H2O	-	Staphylococcus aureus	fluorescein-3'-oxymethyl 2-methoxypropanoate + 2-methoxypropanoate	-	?
fluorescein-3',6'-bis(oxy)methylene bis(2-methoxypropanoate) + H2O	-	Staphylococcus aureus PS 47	fluorescein-3'-oxymethyl 2-methoxypropanoate + 2-methoxypropanoate	-	?
fluorescein-3',6'-bis(oxy)methylene bis(ethoxyacetate) + H2O	-	Staphylococcus aureus	fluorescein-3'-yoxymethyl ethoxyacetate + ethoxyacetate	-	?
fluorescein-3',6'-bis(oxy)methylene bis(ethoxyacetate) + H2O	-	Staphylococcus aureus PS 47	fluorescein-3'-yoxymethyl ethoxyacetate + ethoxyacetate	-	?
fluorescein-3',6'-bis(oxy)methylene bis(methoxyacetate) + H2O	-	Staphylococcus aureus	fluorescein-3'-oxymethyl methoxyacetate + methoxyacetate	-	?
fluorescein-3',6'-bis(oxy)methylene bis(propoxyacetate) + H2O	-	Staphylococcus aureus	fluorescein-3'-oxymethyl propoxyacetate + propoxyacetate	-	?
fluorescein-3',6'-bis(oxy)methylene bis[(methylsulfanyl)acetate] + H2O	-	Staphylococcus aureus	fluorescein-3'-oxymethyl (methylsulfanyl)acetate + (methylsulfanyl)acetate	-	?
fluorescein-3',6'-bis(oxy)methylene bis[[(propan-2-yl)sulfanyl]acetate] + H2O	-	Staphylococcus aureus	fluorescein-3'-oxymethyl [(propan-2-yl)sulfanyl]acetate + [(propan-2-yl)sulfanyl]acetate	-	?
fluorescein-3',6'-bis(oxy)methylene dibutanoate + H2O	-	Staphylococcus aureus	fluorescein-3'-oxymethyl butanoate + butanoate	-	?
fluorescein-3',6'-bis(oxy)methylene dihexanoate + H2O	-	Staphylococcus aureus	fluorescein-3'-oxymethyl hexanoate + hexanoate	-	?
fluorescein-3',6'-bis(oxy)methylene dipentanoate + H2O	-	Staphylococcus aureus	fluorescein-3'-oxymethyl pentanoate + pentanoate	-	?
additional information	enzyme shows modest activity with substrates 4-nitrophenyl acetate, 4-nitrophenyl butanoate, but not with 4-nitrophenyl trimethylacetate	Staphylococcus aureus	?	-	-
additional information	enzyme shows modest activity with substrates 4-nitrophenyl acetate, 4-nitrophenyl butanoate, but not with 4-nitrophenyl trimethylacetate	Staphylococcus aureus PS 47	?	-	-

Subunits

Subunits	Comment	Organism
?	x * 29500, calculated from sequence and SDS-PAGE	Staphylococcus aureus

Synonyms

Synonyms	Comment	Organism
FrmB	-	Staphylococcus aureus
SAOUHSC_02962	-	Staphylococcus aureus

General Information

General Information	Comment	Organism
metabolism	FrmB activates the prodrug bis(pivaloyloxymethyl) (1-hydroxy-2-oxopiperidin-3-yl)phosphonate. Mutation of FrmB renders Staphylococcus aureus sensitive to bis(pivaloyloxymethyl) (1-hydroxy-2-oxopiperidin-3-yl)phosphonate	Staphylococcus aureus
physiological function	FrmB has the highest activity toward oxygen ethers. FrmB hydrolyzes unbranched substrates with little regard for chain length or the end-of-chain bulk within the tested substrates. Branching at the position following the ester carbonyl is deleterious to FrmB activity. When oxygen is included in the chain, positioning at the beta-position to the carbonyl is strongly preferred over the gamma-position	Staphylococcus aureus

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Release 2023.1 (January 2023)